RespiFinder® SMART 22
- Adenovirus (AdV)
- Bocavirus (BoV)
- Bordetella pertussis (B. pertussis)
- Chlamydophila pneumoniae (C. pneumoniae)
- Coronavirus 229E (Cor-229E)
- Coronavirus HKU1 (Cor-HKU1)
- Coronavirus NL63 (Cor-NL63)
- Coronavirus OC43 (Cor-OC43)
- Human Metapneumovirus (hMPV)
- Influenza A (InfA)
- Influenza A/H1N1v (InfA/H1N1v)
- Influenza B (InfB)
- Legionella pneumophila (L. pneumophila)
- Mycoplasma pneumoniae (M. pneumoniae)
- Parainfluenza Type 1 (PIV-1)
- Parainfluenza Type 2 (PIV-2)
- Parainfluenza Type 3 (PIV-3)
- Parainfluenza Type 4 (PIV-4)
- Respiratory Syncytial Virus A (RSVA)
- Respiratory Syncytial Virus B (RSVB)
- Rhinovirus/Enterovirus (RhV/EV)
The RespiFinder® SMART 22 is a multiplex PCR tests which can detect and differentiate 16 RNA viruses, 2 DNA viruses as well as 4 bacteria in a single assay. The RespiFinder® SMART 22 combines the sensitivity and specificity of our MultiFinder technology with the ease of use and accessibility of RealTime PCR.
RespiFinder® SMART 22 is compatible with the LightCycler 480 (Instrument II, Roche), Rotor-Gene 3000/6000 (Corbett) and Rotor-Gene Q (Qiagen) RealTime PCR instruments.
- 18 viral + 4 bacterial pathogens in one assay
- As sensitive as monoplex Real Time PCR
- Contains a competitive internal amplification control
- Diagnosis within 6 hours
- Validated on QCMD panels
- CE-IVD labelled
Acute respiratory tract infection (RTI) is the most widespread type of acute infection in adults and children and is a significant cause of disease in immunocompromised patients. Both viruses and bacteria can cause acute RTI, and the number of causative agents is large as well as diverse. Multiparameter tests provide a fast and accurate means of molecular diagnostics.
The RespiFinder® SMART 22 is a multiplex PCR tests which can detect and differentiate 16 RNA viruses, 2 DNA viruses as well as 4 bacteria in a single assay.
The RespiFinder® SMART 22 assay is based on our proprietary MultiFinder technology, which allows a highly complex analysis of up to 25 targets in a single reaction. After a gene-specific multiplex reverse transcription step, the sample is split into two tubes. Two unique MultiFinder probes are hybridized to the (c)DNA of each pathogen present in the clinical specimen. Hybridized probes are then connected by a ligase enzyme. Subsequently, the ligated probes are amplified using a universal PCR primer pair and detected by melting curve analysis on LightCycler 480 (Roche) or Rotorgene Q/3000/6000 (Qiagen/Corbett) RealTime PCR systems. The amplification and detection of the probes is performed in a single closed tube.
- Nasopharyngeal aspirate/lavage
- swabs
- bronchoalveolar lavage (BAL)
- sputa
The Internal Amplification Control is a RNA transcript from the encephalomyocarditis (EMC) virus and is supplied as a control for the RNA/DNA isolation, the RespiFinder® SMART 22 assay and to check for possible PCR inhibitors.
Detection of the various RespiFinder® SMART amplicons is accomplished by melting curve analysis on LightCycler 480 (Instrument II, Roche), Rotor-Gene 3000/6000 (Corbett) and Rotor-Gene Q (Qiagen) RealTime PCR instruments.
Quality Control for Molecular Diagnostics (QCMD) is an international External Quality Assessment (EQA) provider for molecular diagnosis of infectious agents. PathoFinder has successfully participated in many EQA programs for respiratory pathogens. Details of these tests are available on request. We will keep participating in QCMD programs to ensure the quality, sensitivity and specificity our products.
- 18 viral + 4 bacterial pathogens in one assay
- As sensitive as monoplex Real Time PCR
- Contains a competitive internal amplification control
- Diagnosis within 6 hours
- Validated on QCMD panels
- CE-IVD labelled
| Attachment | Size |
|---|---|
| RespiFinder SMART 22 brochure | 1.55 MB |
