RealAccurate™ M. pneumoniae + C. pneumoniae Duplex PCR kit

Product image:

Summary:

The RealAccurate^TM M. pneumoniae + C. pneumoniae
Duplex PCR kit contains primers and probes to
simultaneously detect Mycoplasma pneumoniae and
Chlamydophila pneumoniae in respiratory samples.

Amplification and detection can be performed on Real Time PCR instruments able to detect fluorescence in the FAM- and in the VIC/YY (Yakima Yellow)-channels.

Contains positive and internal controls
Diagnosis within 2 hours
Validated on QCMD panels
CE labelled
Compatible with RealAccurateTM Respiratory RT PCR kit

Catalog number:

PF0030-R

Overview:

Acute respiratory tract infection is the most widespread type of acute infection in adults and children. The number of pathogens involved is numerous. Respiratory tract infections (RTI) are commonly divided into upper respiratory tract infections (URTI) and lower respiratory tract infections (LRTI). The URTI include rhinorrhea, conjunctivitis, pharyngitis, otis media and sinusitis and LRTI include pneumoniae, brochiolitis and bronchitis. Both viruses and bacteria cause acute RTI.

The RealAccurate^TM M. pneumoniae + C. pneumoniae Duplex PCR Kit is a real time PCR test enabling detection of 2 bacteria that can cause respiratory tract infections.

Procedure:

The RealAccurate^TM M. pneumoniae + C. pneumoniae Duplex PCR Kit is a ready to use optimized reagent containing primers and a FAM-labeled probe for the detection of M. pneumoniae as well as a YY-labeled probe to detect C. pneumoniae DNA. Enzymes are not provided.

Clinical specimens:

Nasopharyngeal aspirate/lavage
swabs
Bronchoalveolar lavage (BAL)

Internal control:

The Internal Control DNA is a DNA fragment from the encephalomyocarditis (EMC) virus and is supplied as a control for the DNA isolation and to check for possible PCR inhibitors.

Detection:

Amplification and detection can be performed on Real Time PCR instruments able to detect fluorescence in the FAM- and in the VIC/YY-channels.

Results image:

QCMD validation:

Quality Control for Molecular Diagnostics (QCMD) is an international External Quality Assessment (EQA) provider for molecular diagnosis of infectious agents.

PathoFinder has successfully participated in many EQA programs for respiratory pathogens in 2007 and 2008. The results of the 2008 CP.MP panel were 100% concordant with the expected results. Details of these tests are available on request.

We will keep participating in QCMD programs to ensure the quality, sensitivity and specificity our products.

Benefits:

Contains positive and internal controls
Diagnosis within 2 hours
Validated on QCMD panels
CE labelled
Compatible with RealAccurate^TM Respiratory RT PCR kit